江西农业学报2011，Vol.23Issue(3)：156-158,164,4.
鹅细小病毒PCR诊断方法的初步建立
Preliminary Establishment of PCR Method for Detection of Goose Parvovirus
摘要
Abstract
DNA was extracted from the tissue of the dead goose caused by the attack of Goose Parvovirus (GPV) as well as from allantoic liquid GPV, a pair of primers were designed according to the sequence of GPV published in GenBank, and an expected 440 bp segment was amplified by PCR. The sequencing result showed that the homology was 99％ in comparison with the reference sequence of GPV strain B which was published in GenBank. This test showed that this PCR method could specially detect GPV and was more sensitive in detecting GPV than AGP assay method. The least amount of GPV that could be detected was 102.5 GELD50/0.2mL.Using this PCR method to detect the distribution of GPV in goose challenged by GPV, the result indicated that GPV existed in the viscera, such as brain, liver, lung, kidney and intestine. All above results reveal that this PCR method for the detection of GPV has been established successfully, and it has strong speciality, high sensitivity and good repetition.关键词
鹅/鹅细小病毒/PCR/琼脂扩散试验分类
农业科技引用本文复制引用
刘宇卓,李银,魏雪涛,张敬峰..鹅细小病毒PCR诊断方法的初步建立[J].江西农业学报,2011,23(3):156-158,164,4.基金项目
江苏省农业科技自主创新资金项目(CX(09)619). （CX(09）
