中药材2011，Vol.34Issue(3)：400-403,4.
龟板提取物对PC12细胞凋亡的影响及其机制
Effects and Mechanism of Plastrum testudinis Extracts on PC12 Apoptosis
摘要
Abstract
Objective :To observe the inhibitive effects of Plastrum testudinis Extracts(PTE) on 6-Hydroxydopamine (6-OHDA)induced PC12 cells apoptosis and explore its mechanism. Methods: PC12 apoptosis model was established by serum starvation and damaged for 24 hours. The cells were randomly divided into four groups: control group,6-OHDA group, PTE 3,30 μg/mL group. Cell optical density was determined by MTT; Ratio of cell apoptosis was examined by Annexin V/PI double stain flow cytometry (FCM), and Western blot was applied to detect the BCL-X/L expression. Results:MTT and FCM analysis demonstrated that PTE can elevate PC12 cells viability and reduce their apoptotic ratio in a dose dependent manner. Western blot showed that PTE promoted the expression of BCL-X/L Conclusion:PTE can inhibit the apoptosis of PC12 induced by 6-OHDA in a dose dependent manner,and its mechanism maybe associated partially with up-regulating BCL-X/L signaling pathway.关键词
龟板提取物/BCL-X/L/PC12细胞/凋亡分类
医药卫生引用本文复制引用
刘洋,伍艺灵,曹佳会,陈东风,周健洪,邓汝东..龟板提取物对PC12细胞凋亡的影响及其机制[J].中药材,2011,34(3):400-403,4.基金项目
国家自然科学基金资助项目(30772861) （30772861）
