首页|期刊导航|中华医学杂志（英文版）|Regulation of epithelial sodium channel a-subunit expression by adenosine receptor A2a in alveolar epithelial cells
中华医学杂志（英文版）2011，Vol.124Issue(10)：1551-1555,5.DOI:10.3760/cma.j.issn.0366-6999.2011.10.021
Regulation of epithelial sodium channel a-subunit expression by adenosine receptor A2a in alveolar epithelial cells
Regulation of epithelial sodium channel a-subunit expression by adenosine receptor A2a in alveolar epithelial cells
摘要
Abstract
Background The amiloride-sensitive epithelial sodium channel a-subunit (a-ENaC) is an important factor for alveolar fluid clearance during acute lung injury. The relationship between adenosine receptor A2a (A2aAR) expressed in alveolar epithelial cells and aα-ENaC is poorly understood. We targeted the A2aAR in this study to investigate its role in the expression of αa-ENaC and in acute lung injury.Methods A549 cells were incubated with different concentrations of A2aAR agonist CGS-21680 and with 100 μmol/L CGS-21680 for various times. Rats were treated with lipopolysaccharide (LPS) after CGS-21680 was injected. Animals were sacrificed and tissue was harvested for evaluation of lung injury by analysis of the lung wet-to-dry weight ratio, lung permeability and myeloperoxidase activity. RT-PCR and Western blotting were used to determine the mRNA and protein expression levels of α-ENaC in A549 cells and alveolar type II epithelial cells.Results Both mRNA and protein levels of α-ENaC were markedly higher from 4 hours to 24 hours after exposure to 100μmol/L CGS-21680. There were significant changes from 0.1 umol/L to 100 μmol/L CGS-21680, with a positive correlation between increased concentrations of CGS-21680 and expression of α-ENaC. Treatment with CGS-21680during LPS induced lung injury protected the lung and promoted α-ENaC expression in the alveolar epithelial cells.Conclusion Activation of A2aAR has a protective effect during the lung injury, which may be beneficial to the prognosis of acute lung injury.关键词
adenosine receptor A2a/epithelial sodium channel a-subunit/acute lung injury/lipopolysaccharideKey words
adenosine receptor A2a/epithelial sodium channel a-subunit/acute lung injury/lipopolysaccharide引用本文复制引用
DENG Wang,WANG Dao-xin,ZHANG Wei ,LI Chang-yi..Regulation of epithelial sodium channel a-subunit expression by adenosine receptor A2a in alveolar epithelial cells[J].中华医学杂志（英文版）,2011,124(10):1551-1555,5.基金项目
This study was supported by a grant from the National Natural Science Foundation of China (No.30971303). （No.30971303）
