中国比较医学杂志2011，Vol.21Issue(4)：1-5,5.DOI:10.3969/j.issn.1671.7856.2011.04.001
体内CD8+T细胞剔除对无症状期SHIV感染猴的影响
Effect of CD8+T Cell Depletion in Vivo on Monkeys with Chronic SHIV-KB9 Infection
摘要
Abstract
Objective CD8 + T cells play an important role in some viral diseases, but the role of CD8 + T cells is not yet fully clear in the asymptomatic phase of HIV infection. The aim of this study was to elucidate the impact of CD8 + T cell in SHIV-infected monkeys with in vivo CD8 + T cells depletion, and to further understand the pathogenesis of AIDS.Methods Eight SHIV-infected rhesus monkeys in the asymptomatic phase were selected and randomly divided into two groups. Anti-CD8 + T antibody cM-T807 were injected intravenously into four monkeys on days 0, 3, and 7. Then the CD8 + T cell number in peripheral blood and lymph nodes in the monkeys were detected by flow cytometry, the plasma viral load by real-time RT-PCR, and the cellular immunity by IFN-γ Elispot assay. Results The viral load of the four monkeys was elevated to above of detected after the CD8 + T cell depletion, but the reactions were not uniform. CD4+ T cells, the HIV-1 target cells, rebound and dropped slightly. The infection of monkeys with CD8 + T cell depletion was lighter than that after the SHIV virus first infection (plasma viral load and CD4 cells ), which was consistent with the Elispot results.Conclusions CD8 + T cells play an important role in the asymptomatic phase of HIV-1 infection, but with some interindividual variation.关键词
SHIV/恒河猴/CD8+T细胞/模型,动物/无症状期分类
医药卫生引用本文复制引用
王卫,丛喆,刘浩,陶真,刘强,魏强,陈志伟,秦川..体内CD8+T细胞剔除对无症状期SHIV感染猴的影响[J].中国比较医学杂志,2011,21(4):1-5,5.基金项目
科技重大专项-艾滋病和病毒性肝炎等重大传染病防治(2009ZX10004-402,008ZX10001-002),中央级公益性科研院所基本科研业务费专项资金(DWS 201009). （2009ZX10004-402,008ZX10001-002）
