中国肺癌杂志2011，Vol.14Issue(4)：311-316,6.DOI:10.3779/j.issn.1009-3419.2011.04.02
Src蛋白在肺癌细胞增殖浸润中的作用
Effects of Src on Proliferation and Invasion of Lung Cancer Cells
摘要
Abstract
Background and objective It has been proven that Src played pivotal roles in carcinogenesis, cancer progression and metastasis.The aim of this study is to explore the roles of Src phosphorylation on lung cancer cells.Methods Western blot and immunoprecipitation was used to detect the expression and phosphorylation of Src in lung cancer cells.MTT and Boyden chamber assay was used to examine the effects of inhibition of Src phosphorylation on proliferation and invasion of lung cancer cells in vitro, respectively.Results pp60src was expressed in alllung cancer celllines in this study All s non-small cell lung cancer (NSCLC) cell lines had increased autophosphorylated tyrosine-418, while nearly no phosphorylated Src in small cell lung cancer SBC5 cell line was detected.The effect of inhibition of Src tyrosine kinase on cell proliferation varied among the lung cancer cell lines.Submicromolar Src tyrosine kinase inhibitor (≤1μM) remarkably suppressed the proliferation of PC-9 and A549 cells in a dose dependent manner (P＜0.05), while the same concentration of Src tyrosine kinase inhibitor had no significant effect on proliferation of H226, PCl4PE6 and RERFLCOK cells.Invasiveness of lung cancer cells was significantly suppressed by Src tyrosine kinase in a dose-dependent manner (P＜0.05).Conclusion Phosphorylation of Src, but not over-expression, plays a pivotal role in proliferation and invasion of NSCLC cell lines in vitro.关键词
肺肿瘤/Src酪氨酸激酶/增殖/浸润Key words
Lung neoplasms/ Src tyrosine kinse/ Proliferation/ Invasion分类
医药卫生引用本文复制引用
郑锐,秦晓松,李文洁,康健..Src蛋白在肺癌细胞增殖浸润中的作用[J].中国肺癌杂志,2011,14(4):311-316,6.基金项目
本研究受辽宁省教育厅高等学校科研项目(No.20061011)、辽宁省自然科学基金项目(No.20082076)和辽宁省科技厅科学技术计划项目(No.2010225028)资助 （No.20061011）
