湖南中医药大学学报2011，Vol.31Issue(3)：27-30,4.
补肾活血方对PCO小鼠未成熟卵母细胞体外核成熟的影响
Effects of Bushen Huoxue decoction on in-vitro nuclear maturation of immature PCO oocytes in mice
摘要
Abstract
Objective To investigate the effect of Bushen Huoxue decoction on in-vitro nuclear maturation of immature PCO oocytes in mice.Methods (1) The serum containing Bushen Huoxue decoction was prepared.(2)The mice models with polycystic ovary were made.(3)The immature oocytes isolated from the polycystic ovaries of the mice in the germinal vesicle (GV) were cultured with the serum containing decoction collected from different time respectively and the relation of GVBD and PB1 extrusion were detected.Results (1)GVBD after 2h and 2.5h were higher in the serum containing decoction group than that in the controls and normal serum groups, and the difference was the most notable after 4th hours (P＜0.01).(2) The PB1 in 2 h and 2.5 h in the serum groups were higher than that in the controls and normal group, and the difference was the most notable after 18th hours.Conclusion The serum containing Bushen Huoxue decoction after 2～2.5 h is very effective to promote in-vitro nuclear maturation of immature oocytes significantly in PCO mice.关键词
补肾活血方/血清/卵母细胞/生发泡破裂/第一极体/鼠/紫石英/桑寄生/泽泻Key words
Bushen Huoxue decoction/ serum/ oocyte/ germinal vesicle breakdown/ PBl/ mice/ fluoritum/ paraphyton/ rhizoma alismatis分类
医药卫生引用本文复制引用
孙晓峰,尤昭玲,刘丹卓,付灵梅,龚力民,吴霞..补肾活血方对PCO小鼠未成熟卵母细胞体外核成熟的影响[J].湖南中医药大学学报,2011,31(3):27-30,4.基金项目
湖南省科技厅博士后专项(2009RS3018) （2009RS3018）
湖南省教育厅资助项目(2009C727). （2009C727）
