首页|期刊导航|中华医学杂志（英文版）|Human umbilical cord mesenchymal stem cells derived from Wharton's jelly differentiate into insulin-producing cells in vitro
中华医学杂志（英文版）2011，Vol.124Issue(10)：1534-1539,6.DOI:10.3760/cma.j.issn.0366-6999.2011.10.018
Human umbilical cord mesenchymal stem cells derived from Wharton's jelly differentiate into insulin-producing cells in vitro
Human umbilical cord mesenchymal stem cells derived from Wharton's jelly differentiate into insulin-producing cells in vitro
摘要
Abstract
Background Islet transplantation is an effective way of reversing type Ⅰ diabetes. However, islet transplantation is hampered by issues such as immune rejection and shortage of donor islets. Mesenchymal stem cells can differentiate into insulin-producing cells. However, the potential of human umbilical cord mesenchymal stem cells (huMSCs) to become insulin-producing cells remains undetermined.Methods We isolated and induced cultured huMSCs under islet cell culture conditions. The response of huMSCs were monitored under an inverted phase contrast microscope. Immunocytochemical and immunofluorescence staining methods were used to measure insulin and glucagon protein levels. Reverse transcription-polymerase chain reaction (RT-PCR) was performed to detect gene expression of human insulin and PDX-1. Dithizone-staining was employed to determine the zinc contents in huMSCs. Insulin secretion was also evaluated through radioimmunoassay.Results HuMSCs induced by nicotinamide and β-mercaptoethanol or by neurogenic differentiation 1 gene (NeuroD1)transfection gradually changed morphology from typically elongated fibroblast-shaped cells to round cells. They had a tendency to form clusters. Immunocytochemical studies showed positive expression of human insulin and glucagon in these cells in response to induction. RT-PCR experiments found that huMSCs expressed insulin and PDX-1 genes following induction and dithizone stained the cytoplasm of huMSCs a brownish red color after induction. Insulin secretion in induced huMSCs was significantly elevated compared with the control group (t=6.183, P＜0.05).Conclusions HuMSCs are able to differentiate into insulin-producing cells in vitro. The potential use of huMSCs in βcell replacement therapy of diabetes needs to be studied further.关键词
human umbilical cord/mesenchymal stem cells/differentiation/insulin-producing cellsKey words
human umbilical cord/mesenchymal stem cells/differentiation/insulin-producing cells引用本文复制引用
WANG Hong-wu,LIN Li-min,HE Hong-yan,YOU Fang,LI Wei-zhong,HUANG Tian-hua,MA Gui-xia,MA Lian..Human umbilical cord mesenchymal stem cells derived from Wharton's jelly differentiate into insulin-producing cells in vitro[J].中华医学杂志（英文版）,2011,124(10):1534-1539,6.基金项目
This work was supported by the grants from Science and Technology Program Foundation of Guangdong Province,China (No.2010B031600273 and No.2008B030301237),the Science and Technology Program Foundation of Shantou,Guangdong Province,China (No.E200900137) and the Foundation of Department of Health,Guangdong Province,China (No.B2010223). （No.2010B031600273 and No.2008B030301237）
