安徽农业大学学报2011，Vol.38Issue(2)：296-298,3.DOI:CNKI:34-1162/S.20110303.0940.006
采用ASPCR技术检测抗药性雨久花ALS突变基因碱基种类的探讨
Development of allele-specific PCR for detection of herbicide-resistance in Monochoria korsakowii Regel et Maack
摘要
Abstract
Allele-specific PCR is a rapid, simple, and low-cost method for single-nucleotide polymorphism identification. Using this technique, a single-nucleotide polymorphism primer marker of thymine(susceptible) or guanine(resistant) at the 3'end on 592 point of ALS(acetolactate synthetase) gene encoding site (197 point of ALS amino acid) was designed to detect the herbicide-resistant type of Monochoria korsakowii Regel et Maack. in Yanbian region. The result shows that susceptibility primer marker can be used to detect the susceptibility biotype;otherwise can be detected resistant biotype; the cause of resistance to sulfonylurea herbicide type can be induced by substitution of Pro197 to histidine. It proved that the technique was efficient and feasible to detect ALS gene of herbicide-resistant type.关键词
等位基因特异性扩增/雨久花/乙酰乳酸合成酶基因/抗药性/检测Key words
Allele-specific PCR/ Monochoria korsakowii Regel et Maack/ ALS gene/ herbicide-resistance/detection分类
农业科技引用本文复制引用
吴明根,金万赫,许勇男..采用ASPCR技术检测抗药性雨久花ALS突变基因碱基种类的探讨[J].安徽农业大学学报,2011,38(2):296-298,3.基金项目
延边大学科学基金项目资助. （）
