中国当代儿科杂志2011，Vol.13Issue(5)：406-410,5.
Galectin-9及其受体Tim-3在哮喘小鼠肺组织中的表达
Expression of Galectin-9 and Tim-3 in lungs of mice with asthma
张志英 1栾斌 1冯晓霞1
作者信息
- 1. 郑州大学第三附属医院儿内科,河南,郑州,450052
- 折叠
摘要
Abstract
Objective To study the expression of Galectin-9 and Tim-3 in lungs of mice with asthma and the effect of rosiglitazone (PPAR-γagonist) on their expression.Methods Forty-five BALB/c SPF female mice were randomized into control group and asthma groups with and without rosiglitazone intervention.After ovalbumin stimulation and rosiglitazone intervention the pathological changes of the lung tissues were observed.Galectin-9 and Tim-3 mRNA levels in lung tissues were determined using RT-PCR.The levels of IL-4 and IFN-γ in peripheral blood were measured using ELISA.Results The expression of Galectin-9 and Tim-3 mRNA of lung tissues in the untreated asthma group increased significantly compared with the control and the rosiglitazone treated groups ( P ＜ 0.05 ).A significantly increased blood expression of IL-4 and a significantly decreased blood expression of IFN-γwere found in the untreated asthma group compared with the control and the rosiglitazone-treated groups ( P ＜ 0.05 ).The expression of Galectin-9 and Tim-3 mRNA was positively correlated with blood IL-4 level ( r = 0.792, r = 0.794 respectively; P ＜ 0.05 ), but negatively correlated with blood IFN-γlevel ( r = - 0.692, r = - 0.757 respectively; P ＜ 0.05 ).Conclusions Galectin-9 and Tim-3 mRNA levels in lungs increase in mice with asthma and significantly correlate with the levels of blood Thl/Th2 eytokines.This suggests that Galectin-9 and Tim-3 are closely related to inflammatory process in asthma.Rosiglitazone treatment may decrease the expression of Galectin-9 and Tim-3.关键词
哮喘/半乳糖凝集素-9/T细胞免疫球蛋白粘蛋白分子-3/小鼠分类
医药卫生引用本文复制引用
张志英,栾斌,冯晓霞..Galectin-9及其受体Tim-3在哮喘小鼠肺组织中的表达[J].中国当代儿科杂志,2011,13(5):406-410,5.
