中国当代儿科杂志2011，Vol.13Issue(5)：424-427,4.
PI3K/Akt信号通路对新生大鼠缺氧缺血性脑损伤后学习记忆能力的影响
Effects of PI3 K/Akt signaling pathway on learning and memory abilities in neonatal rats with hypoxic-ischemic brain damage
姚丹 1何雪 1王金湖 1赵正言1
作者信息
- 1. 浙江大学医学院附属儿童医院儿童保健科,浙江,杭州,310003
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摘要
Abstract
Objective To study the effects of PI3K/Akt signaling pathway inhibitor wortmannin on long-term learning and memory abilities in neonatal rats with hypoxic-ischemic brain damage (HIBD). Methods Forty-eight neonatal rats were randomly assigned to blank control ( n = 8 ), sham-operated ( n = 8 ), HIBD model ( n = 10), HIBD + DMSO (dimethyl sulfoxide, n = 8 ) and HIBD + wortmannin groups (n = 8 ). Wortmannin (2 μL) was injected to the left hippocampus 30 minutes before HIBD inducement in the HIBD + wortmannin group. The Morris water maze test was used to examine the long-term learning and memory abilities at the age of 28 days. Results With the increased number of swimming, the escape latency was shortened in various groups. From the second day, the escape latency in the HIBD + wortmannin group was significantly longer than that in the sham-operated and the blank control groups ( P ＜ 0. 05 ), and the differences increased with the time. On the fourth day, there were significant differences in the escape latency between the HIBD + wortmannin group and the HIBD + DMSO group as well as the HIBD model group ( P ＜ 0. 05 ). On the eighth day (retention trial), there were the most obvious differences in the escape latency between the HIBD + wortmannin group with the other four groups. In the space exploration test, the number of times crossing the former platform location within 120 seconds after removing the platform in the HIBD + DMSO and the HIBD model group was lower than the sham-operated and the blank control groups ( P ＜ 0. 05 ). The HIBD + wortmannin group showed lower number of times crossing the former platform location compared with the HIBD + DMSO and the HIBD model groups ( P ＜ 0.05 ), as well as the sham-operated and the blank control groups (P ＜ 0. 01 ). Conclusions PI3K/Akt signaling pathway inhibitor wortmannin can aggravate the cognitive impairments,thus affecting adversely long-term learning and memory abilities in neonatal rats with HIBD.关键词
缺氧缺血性脑损伤/PI3K/Akt/Morris水迷宫/学习记忆/新生大鼠分类
医药卫生引用本文复制引用
姚丹,何雪,王金湖,赵正言..PI3K/Akt信号通路对新生大鼠缺氧缺血性脑损伤后学习记忆能力的影响[J].中国当代儿科杂志,2011,13(5):424-427,4.
