中国比较医学杂志2011，Vol.21Issue(5)：22-25,4.DOI:10.3969/j.issn.1671.7856.2011.05.006
辽宁省6种常用近交系小鼠10个微卫星位点的遗传质量检测报告
A Genetic Monitoring Report for Ten Microsatellites DNA Locis of Six Inbred Mice Strains Used in Liaoning Province
韩喜彬 1苏玉虹 2巴彩凤 1贺宝军1
作者信息
- 1. 辽宁医学院实验动物中心,锦州,121001
- 2. 辽宁医学院畜牧兽医学院,锦州,121001
- 折叠
摘要
Abstract
Objective To study the genetic quality for six strains of inbred mice in Liaoning Province by microsatellites technology. Methods Ten microsatellites DNA locis and primers were selected in Mouse Genome Database and pertinent literature, and its polymorphism information content was rich, to analyzed on genetic polymorphism of mouse by PCR Amplification and PAGE electrophoresis. Results The same site results of PCR amplification showed polymorphism in different strains, and showed monomorphism in same strains, ten microsatellites DNA locis were homozygous state for all mouse. The genetic distance among C57BL/10 and C57BL/6J was 0. 1021, and it was 0. 1635 and 0.1614 between BALB/c with C57BL/10 and C57BL/6J. Conclusions It was a feasible way to analyzed genetic monitoring among inbred mouse strains by ten microsatellites DNA locis.关键词
近交系小鼠/遗传质量检测/微卫星分类
生物科学引用本文复制引用
韩喜彬,苏玉虹,巴彩凤,贺宝军..辽宁省6种常用近交系小鼠10个微卫星位点的遗传质量检测报告[J].中国比较医学杂志,2011,21(5):22-25,4.
