中国比较医学杂志2011，Vol.21Issue(5)：45-48,封3,5.DOI:10.3969/j.issn.1671-7856.2011.05.011
多种特殊染色法在骨关节炎组织形态学研究中的应用比较
Comparison of Several Special Stainings on Osteoarthritis Morphology
摘要
Abstract
Objective To investigate the staining rule of several special staining methods on bone and joint staining and their value in morphological research of osteoarthritis.Methods 20 6-month old healthy New Zealand rabbits were randomly divided into normal group and osteoarthritis model group, 10 in each group.The model rabbits were replicated into knee osteoarthritis model by modified Hulth method.The materials were drawn out 6 weeks after operation.The samples were fixed, decalcified, paraffin embedded and made into paraffin sections, then HE staining, Safranin-Fast Green staining, AB-PAS staining, toluidine blue staining, Van Gieson staining and Mallory staining were applied to observe the morphology of the tissue, and all of the staining methods were compared.Results HE staining showed normal tissue morphology of joints, pathological changes of osteoarthritis can be seen in articular cartilage and subchondral bone of model group; Safranin-Fast Green staining showed clear boundary of cartilage and subchondral bone (cement line) , and a clear tide line, the glycosaminoglycan in Cartilage matrix decreased and the fiber Increased; AB-PAS staining showed that glycosaminoglycan, especially the acid glycosaminoglycan reduced in osteoarthritis; toluidine blue staining showed the acid glycosaminoglycan reduced in the tissues of osteoarthritis; Van Gieson staining and Mallory staining showed collagen fibers of bone and joint tissues, but the boundaries of various tissues were not clear.Conclusion Application of HE staining,in combination with safranin-fast green staining and AB-PAS staining in osteoarthritis study may acquire more objective and comprehensive information on joint morphology.关键词
骨关节炎/特殊染色法/兔分类
社会科学引用本文复制引用
黄云梅,陈文列,黄美雅,林如辉,吴广文,李钻芳..多种特殊染色法在骨关节炎组织形态学研究中的应用比较[J].中国比较医学杂志,2011,21(5):45-48,封3,5.基金项目
福建省自然科学基金项目(NO.C0710030) （NO.C0710030）
陈可冀中西医结合发展基金项目(CKJ2008008,ZXY2008004) （CKJ2008008,ZXY2008004）
福建省重点实验室开放课题基金、陈可冀中西医结合发展基金项目(2008J1004-29,CKJ2008066). （2008J1004-29,CKJ2008066）
