植物学报2011，Vol.46Issue(3)：276-284,9.DOI:10.3724/SP.J.1259.2011.00276
锦葵科植物DNA条形码通用序列的筛选
Screening of Universal DNA Barcodes for Malvaceae Plants
摘要
Abstract
To screen the best candidate sequence that can be used as a universal DNA barcode to identify species in Malvaceae, we amplified by PCR and sequenced the DNA fragments of 5 hot regions (ITS, ITS2, rbcL, matK and psbA-trnH) in samples from the family Malvaceae. PCR amplification and sequencing, differential intra- and interspecific divergences, DNA barcoding gap and identification (using BLAST1 and nearest distance methods) were used to evaluate the discrimination ability of these candidate sequences. The ITS region had fairly high amplification efficiency in 26 plant samples belonging to 11 Malvaceae species and better intra- and interspecific divergences and DNA barcoding gap than any other region tested (ITS2, psbA-trnH and rbcL). In addition, the rate of successful identification with the ITS region was 89.9％ at the species level for after added 1 228 plant samples from the GenBank database of Malvaceae belonging to 316 species from 60 distinct genera. The psbA-trnH region had the best amplification efficiency and sequencing efficiency; the rate of successful identification with the psbA-trnH region was 63.2％, and the region can discriminate some species that cannot be successfully discriminated by the ITS region. We propose that ITS and psbA-trnH are a promising DNA barcode sequence combination for identification.关键词
DNA条形码/鉴定/ITS/ITS2/锦葵科/psbA-trnHKey words
DNA barcoding/ identification/ ITS/ ITS2/ Malvaceae/ psbA-trnH引用本文复制引用
王柯,陈科力,刘震,陈士林..锦葵科植物DNA条形码通用序列的筛选[J].植物学报,2011,46(3):276-284,9.基金项目
国际科技合作项目(No.2007DFA30990)和卫生行业科研专项(No.200802043) （No.2007DFA30990）
