植物学报2011，Vol.46Issue(3)：285-292,8.DOI:10.3724/SP.J.1259.2011.00285
甘露醇对拟南芥基因组DNA甲基化的影响
Methylation-sensitive Amplified Polymorphism Analysis of DNA Methylation in Arabidopsis Under Mannitol Treatment
摘要
Abstract
We aimed to assess the effect of mannitol treatment on plant growth, as well as genomic DNA methylation levels and patterns in Arabidopsis thaliana by methylation-sensitive amplified polymorphism (MSAP) analysis. Mannitol at 50, 100, 150 and 200 mmol·L-1 promoted the root length of Arabidopsis seedlings. MSAP analysis revealed that levels of 5-methyldeoxycytidine (5-mdC) in mannitol-treated seedlings were also altered significantly, from 17.75％, 21.15％,15.49％ to 46.10％ in plants treated with 50, 100, 150 and 200 mmol·L-1 mannitol, respectively. Thus, mannitol caused epigenetic changes in all samples based on different levels and patterns of DNA methylation. As the controls, methylation and demethylation of DNA in the seeds treated with mannitol was 5.78％, 15.48％, 10.71％, 33.73％ and 10.98％, 5.36％,8.33％, 7.69％, respectively. Mannitol causes a dose-dependent and transient change in global 5-mdC levels in Arabidopsis seedlings.关键词
拟南芥/DNA甲基化/甘露醇/MSAPKey words
Arabidopsis, DNA methylation, mannitol, methylation-sensitive amplified polymorphism引用本文复制引用
杜亚琼,王子成..甘露醇对拟南芥基因组DNA甲基化的影响[J].植物学报,2011,46(3):285-292,8.基金项目
国家自然科学基金(No.30900973) （No.30900973）
