武警医学2011，Vol.22Issue(5)：381-384,4.
人破骨细胞的体外培养及鉴定
Culture and identification of human osteoclasts in vitro
摘要
Abstract
Objective To establish an in vitro method of culture and identification of human osteoclasts, and to provide a large number of highly - active osteoclasts for experimental researches.Methods Mononuclear cells were obtained from bone marrow.The osteoclasts were cultured in different media, by different inducers and at different concentrations.Morphological characteristics of cells were identified by TRAP staining, and specific proteins by ELISA.The ability of resorption was analysed by scanning electron microscopy.Results In group C and D, the a -MEM was used as culture medium and 1 , 25(OH)2VD3( 10-7 mmol/L) or RANKL (50 ng/ml) and M - CSF (30 ng/ml) as inducers.The osteoclasts were multinuclear and highly - active.Conculsions A method has been found to cultivate numerous highly - active human osteoclasts in vitro.关键词
人破骨细胞/细胞培养/TRAP染色Key words
osteoclasts/ cell culture/ TRAP staining分类
临床医学引用本文复制引用
刘水涛,雷鸣,安佰京,杨军,张鹏礼,邢更彦..人破骨细胞的体外培养及鉴定[J].武警医学,2011,22(5):381-384,4.基金项目
武警总医院Ⅰ类课题(WZ 2009052) （WZ 2009052）
