广东医学2011，Vol.32Issue(11)：1402-1405,4.
索拉非尼诱导K562细胞凋亡机制的研究
Mechanism of sorafenib in inducing apoptosis of K562 cells
摘要
Abstract
Objective To observe the effects of sorafenib on proliferation and apoptosis of BCR/ABL positive human chronic myelogenous leukemia cell - line K562. Methods K562 cells were cultured with different concentrations of sorafenib ( 0, 5 , 7. 5 , 10 , 12. 5 , 15 and 20 μmol/L ) for 48 h. Cell viability was determined by CCK - 8 assay. Apoptosis was conducted by AnnexinV/PI, PI staining and Hochest 33342. PARP protein was detected by Western blotting. Results Sorafenib induced inhibition of cell growth, cell apoptosis and PARP cleavage were observed in dose - dependent manners. Conclusion PARP cleavage , a marker of caspase cascades activation, is induced hy sorafenib, suggesting that sorafenih induces K562 apoptosis via this pathway. Thus it provides therapeutic effects of sorafenib on imatinib - resistant chronic myeloid leukemia.关键词
索拉非尼/K562细胞/细胞凋亡/PARPKey words
sorafenib/ K562/ apoptosis/ PARP引用本文复制引用
肖若芝,陈琰,王立琳,何程明,阮星星,熊慕珺 ,林东军..索拉非尼诱导K562细胞凋亡机制的研究[J].广东医学,2011,32(11):1402-1405,4.基金项目
广东省科技计划项目(编号:2006B35501008) （编号:2006B35501008）
