福建师范大学学报（自然科学版）2011，Vol.27Issue(2)：101-108,8.
福建霞浦海区刺激隐核虫虫株鉴定及生活史观察
The Strain and Life-cycle of Cryptocaryon irritans Isolated from Pseudosciaena crocea Cultured in Xiapu, Fujian
摘要
Abstract
A strain of Cryptocaryon irritans was isolated from cultured Pseudosciaena crocea in Xiapu, Fujian, maintained and passaged successfully in aquaria by using Sebastiscus marmoratus as a host. The life cycle and the morphological characters of each phase were observed. Meanwhile, its genomic DNA was extracted and used as a template DNA to amplify the gene fragment of internal transcribed spacers of ribosomal DNA (rDNA-ITS-1) by using PCR. The sequence of rDNA-ITS-1 was then analyzed and aligned with the known homological sequences of various strains of Cryptocaryon irritans. The result showed that both the morphological characterization and the sequence of rDNA-ITS-1 were identical to those of PYH4.12 and Chiayi strain, which inferred that the pathogen of ‘white spot disease’ endemic in cultured Pseudosciaena crocea in Xiapu, Fujian is PYH4.12 or Chiayi strain of Cryptocaryon irritans. It provided with large numbers of the parasites for establishment of cDNA libraries and analysis of function genes.关键词
刺激隐核虫/褐菖鲉/鉴定/传代/生活史Key words
Cryptocaryon irritans / Sebastiscus marmoratus / identification/ passage/ life cycle分类
生物科学引用本文复制引用
孙志宇,郑昌峰,武晓燕,郭果为,汪彦愔,黄晓红..福建霞浦海区刺激隐核虫虫株鉴定及生活史观察[J].福建师范大学学报（自然科学版）,2011,27(2):101-108,8.基金项目
国家自然科学基金资助项目(31040084) （31040084）
福建省自然科学基金重点项目(2008J0004) （2008J0004）
福建师范大学人才建设项目 （）
