中国中医药信息杂志2011，Vol.18Issue(4)：31-33,3.DOI:10.3969/j.issn.1005-5304.2011.04.013
生精散治疗大鼠弱精子症的实验研究
Experimental Study on Shengjingsan Recipe for the Treatment of Asthenozoospermia of Rat Model
摘要
Abstract
Objective To study the effect of Shengjingsan recipe on asthenozoospermia in rat model.Methods Rat model of asthenozoospermia was induced by oral administration of multi-glycosides of tripterygium wilfordii (GTW) everyday for 28 days.The sperm viability and the sperm vitality, sperm concentration as well as the sperm mobility were examined by computer assisted sperm analysis (CASA), and the sperm morphology was detected by hematoxylin-eosin (HE) staining.Rats were randomly divided into normal control group (normal diet, 42 d), NS group (1 mL/d NS, 28 d), GTW group (20 mg/kg GTW, 4 weeks) and small (0.01 g/d)-, medium (0.02 g/d)-, large (0.03 g/d)-dose of Shengjingsan Recipe group (14 d), five rats in each group.Results The parameters of the sperm viability and the sperm vitality, sperm concentration as well as the sperm mobility including straight-line velocity (VSL), curve-line velocity (VCL), and average path velocity (VAP) decreased significantly in GTW group compared with normal control group and NS group.The HE staining of the testicle tissues showed that the spermatocytes and the spermatozoon cells reduced obviously in the seminiferous tubule in GTW group, in which abnormal arrangement of the spermatogenic cells occurred.The above decreased parameters of the sperm viability and the sperm vitality, sperm concentration as well as the sperm mobility, induced by GTW, could be reversed effectively after Shengjingsan recipe treatment at small, medium, and large dosage.Large dose of Shengjingsan recipe ameliorated beat-cross frequency and mean angular displacement of sperm.Conclusions GTW can induce typical asthenozoospermia in rats.Shengjingsan recipe has therapeutic effect on asthenozoospermia by improving the sperm viability and the sperm vitality, sperm concentration as well as the sperm mobility.关键词
弱精子症/生精散/雷公藤多甙/精子运动/大鼠分类
医药卫生引用本文复制引用
韩彩艳,孙伟,王波,王亚男,张斌,蔡捷,邢国刚..生精散治疗大鼠弱精子症的实验研究[J].中国中医药信息杂志,2011,18(4):31-33,3.基金项目
国家自然科学基金(81072951) （81072951）
