应用生态学报2011，Vol.22Issue(5)：1280-1286,7.
共代谢条件下光合细菌对2-氯苯酚的生物降解
Biodegradation of o-chlorophenol by photosynthetic bacteria under co.metabolism
摘要
Abstract
Photosynthetic bacterial strain PSB-ID cannot utilize o-chlorophenol (2-CP) as the sole carbon source for energy. In this paper, different carbon sources ( malic acid, sodium propionate,sodium acetate. sodium citrate, phenol, glucose. and soluble starch) were taken as the co-metabolism substrates to study their effects on PSB-ID growth and 2-CP degradation under the condition of aerobic culture in darkness. Among the substrates, glucose was most efficient, which promoted the reproduction of PSB-ID. enhanced the 2-CP degradation efficiency, and shortened the degradation period. The optimization experiment of added concentration of glucose showed that when the added glucose concentration was 3 g· L-1, the PSB-ID cell concentration AD560 after 168 h culture was 1. 749. the half-time of 2-CP was shortened to 3. 9 d. and the degradation rate constant was increased to 0. 00864 h-1. The SDS-PAGE analysis on the total microbial cellular protein showed that taking glucose as the co-metabolism substrate. PSB-ID could induce a specific 2-CP-degrading enzyme .关键词
光合细菌/2-氯苯酚/共代谢/生物降解/降解动力学分类
资源环境引用本文复制引用
董怡华,胡筱敏,和英滇,李亮..共代谢条件下光合细菌对2-氯苯酚的生物降解[J].应用生态学报,2011,22(5):1280-1286,7.基金项目
国家水体污染控制与治理科技重大专项(2008ZX07208-009)资助. （2008ZX07208-009）
