中国药理学通报2011，Vol.27Issue(7)：934-939,6.DOI:10.3969/j.issn.1001-1978.2011.07.012
革皮氏海参皂苷抑制血管新生作用
Antiangiogenesis effect of holothurin A1 and 24-dehydroechinoside A from Pearsonothria graeffei
摘要
Abstract
Aim To determine the effects of holothurin Al( HA ) and 24-dehvdroechinoside A( DA ) extracted from Pearsonothria graeffe on anti-angiogenesis. Methods Inhibition of angiogenesis was assessed in vitro using proliferation, AO/EB and tubeformation assaysin HA and DA treated HUVEC cells. In vivo, CAM as-says were used to assess inhibitory effect of HA and DAon physiological angiogenesis. Results HA and DAcould inhibit the proliferation of endothelial cells andinduce the apoptosis of HUVEC cells. The ICso were4. 80 μmol·L-1 and 3. 82 μmol · L-1 in HA and DAtreated HUVEC cells for 48 h respectively. HA andDA could inhibit the tube formation in vitro and de-crease the blood vessel growth on the chicken chorioal-lantoic membrane in vivo. The effect of DA was betterthan HA,indicating that the hydroxy substituted on C-22 would decrease the anti-tumor and anti-metastasisactivity of triterpene glycosides and the carbon bondson 24( 25 ) would increase the anti- angiogenesis activ-ity of triterpene glycosides. Conclusion Both HA andDA exert significant anti-angiogenic activities relatingto their chemical structures.关键词
革皮氏海参皂苷/人脐静脉内皮细胞/细胞凋亡/小管形成/黏附/血管新生分类
医药卫生引用本文复制引用
赵芹,薛长湖,张珣,董平,王玉明,王静凤..革皮氏海参皂苷抑制血管新生作用[J].中国药理学通报,2011,27(7):934-939,6.基金项目
国家自然科学基金资助项目(No 30972284) （No 30972284）
国际科技合作项目(No 2010DFA31330) （No 2010DFA31330）
海洋公益性行业科研专项(No 201105029) （No 201105029）
"泰山学者"建设工程专项经费 （）
