天然产物研究与开发2011，Vol.23Issue(2)：208-211,4.
北柴胡不定根系生物转化合成皂苷研究
Biotransformation and Biosynthesis of Saikosaponins in Adventitious Root System of Bupleurum chinense
摘要
Abstract
The conditions and methods of using Bupleurum chinese adventitious root system to produce saikosaponins were studied by weans of adding precursors, accelerators and high sucrose concentrations.The results showed that the addition of proper concentrations of magnesium sulfate, potassium nitrate, sodium pyruvate and sucrose in medium had the effects on biotransformation and biosynthesis of saikosaponins.The most suitable components were 1/2 MS medium adding 0.37 g/L magnesium sulfate,5.7 g/L potassium nitrate,2.0 mmol/L sodium pyruvate and 50 g/L sucrose,in which the content of saikosaponins were enhanced 9.74 times.关键词
北柴胡/不定根/生物转化/柴胡皂苷Key words
Bupleurum chinense DC/ adventitious root/ biotransformation/ saikosaponins分类
农业科技引用本文复制引用
关倩,郝建平,杨东方,韩丹,高可青,周琼..北柴胡不定根系生物转化合成皂苷研究[J].天然产物研究与开发,2011,23(2):208-211,4.基金项目
山西省科技攻关计划项目(20100311091) （20100311091）
