林业科学2011，Vol.47Issue(3)：68-74,7.
青钱柳种子休眠机制
Seed Dormancy Mechanism of Cyclocarya paliurus
摘要
Abstract
Grown for both its fine timber and medicinal use, Cyclocarya paliurus is a monotypic genus of Juglandaceae,and is an endemic plant to China. However, the seeds remain deeply dormant in natural condition. In order to assess the dormancy mechanism of the seeds, a germination test of both isolated embryos and seeds were conducted with different treatments, and water and gas permeability of the husk, as well as the bioassay and identification of extracts from different seed fractions were measured. The results showed that C. paliurus seed scarcely had embryo dormancy, and the dormancy of the seed was mainly attributed to its pericarp and seed coat. The mechanical and permeability barrier of periearp and seed coat had a negative effect on germination to a certain extent, but seed dormancy of C. paliurus was mainly due to the inhibitory substances in the pericarp. The bioassay results indicated that the inhibitors were distributed in all the fractions of the seeds, and the inhibition activity was found as the following: pericarp ＞ seed coat ＞ intact seeds ＞ embryo. Methanol extract of the pericarp was further separated by the method of systemic solvents, and a bioassay of the separated fractions showed that the ether phase had the strongest inhibitory effect. Analysis of GC-MS revealed that the maximal component in ether phase of the periearp was vanillic acid, accounted for 35.09％. The results from this study systematically illustrated the dormancy mechanism of C. paliurus seeds for the first time, and would provide some theoretical bases for breaking the seed dormancy in practice.关键词
青钱柳/种子休眠/离体胚培养/种壳透性/抑制物质Key words
Cyclocarya paliurus/ seed dormancy/ embryo culture in vitro/ permeability of husk/ inhibitors分类
农业科技引用本文复制引用
尚旭岚,徐锡增,方升佐..青钱柳种子休眠机制[J].林业科学,2011,47(3):68-74,7.基金项目
国家林业公益性行业科研专项(200904046) （200904046）
江苏省高等学校创新团队科研计划(苏教科[2009]10号) （苏教科[2009]10号）
江苏省"六大人才高峰"高层次人才项目. （）
