安徽农业科学2011，Vol.39Issue(5)：2804-2806,3.
Asial型口蹄疫病毒P1基因的原核表达及其抗血清的制备
Prokaryotic Expression of P1 gene of Type Asia1 Foot and Mouth Disease Virus (FMDV) and the Preparation of Its Antiserum
摘要
Abstract
[ Objective ] The aim was to study on the prokaryotic expression of P1 gene of type Asial foot and mouth disease virus (FMDV) as well as the preparation of its antiserum. [ Method] The P1 gene of type Asial FMDV was obtained by gene cloning techniques, and then the P1 gene was cloned into pET-32a ( + ) plasmid; subsequently transformed the recombinant plasmid into E. coliBL21 ( DE3), after the IPTG induction and protein purification, SDS-PAGE was carried out; the ultrasonic wave was use to lyse the cultivated recombinant strain, and after the isolation and purification, this fusion protein was utilized to immunize New Zealand rabbits se as to prepare P1 protein antiserum. [ Result] The positive clones were obtained; SDS-PAGE result showed that the target band was appeared at 105 kD; Western blot analysis showed that the antisera could bind to the expressed P1 fusion protein specifically; the ELISA titer of the rabbit anti-FMDV-P1 sera was approximately 1∶5 120. [ Conclusion] This study had provided foundations for FMDV serological diagnostic methods and genetically engineered vaccine.关键词
Asial 型口蹄疫病毒/P1基因/原棱表达/抗血清Key words
Foot-and-mouth disease virus (FMDV)/ P1 gene/ Prokaryotic expression/ Antiserum分类
农业科技引用本文复制引用
武刚,王洪梅,刘晓,王立群,于力,仲跻峰,何洪彬..Asial型口蹄疫病毒P1基因的原核表达及其抗血清的制备[J].安徽农业科学,2011,39(5):2804-2806,3.基金项目
国家转基因重大专项(2009ZX08007-006B) （2009ZX08007-006B）
国家自然科学基金项目(31072160) （31072160）
山东省科技攻关项目(2009GG20002032) （2009GG20002032）
山东省自然基金项目(Y2008D20) （Y2008D20）
兽医生物技术国家重点实验室开放课题基金项目(SKLVBF200806). （SKLVBF200806）
