安徽农业科学2011，Vol.39Issue(5)：2807-2809,2812,4.
蚕蛹壳几丁质降解菌的分离筛选及其产酶条件优化
Isolation and Screening of Silkworm Puparium Chitin Degradation Strains and the Optimization of Chitinase-produce Conditions
摘要
Abstract
[Objective] The aim of this study was to optimize the conditions of chitinase-produce strains. [ Method] A kind of screened chitinase-produce strain G-254 was habituated cultured, and then the single factor experiment was carried out to explore the effects of different carbon source, nitrogen source and inorganic salt on the activity of produced chitinase; the response surface test was used to determine the optimal conditions for chitinase production. [ Result] The optimal conditions for chitinase production were: 8％ of glucose, 5％ of beef extract and 0.07％ of MgSO4, and the activity of chitinase reached the maximal value (6.86 U) under these conditions. [ Conclusion ] The study improved the activity of chitinase produced by strain G-254 and provided good foundation for industrial production.关键词
蚕蛹壳/几丁质/降解/酶活/优化Key words
Silkworm puparium/ Chitin/ Degradation/ Chitinase activity/ Optimization分类
农业科技引用本文复制引用
赵祥杰,邝哲师,叶明强,罗国庆,肖更生,廖森泰..蚕蛹壳几丁质降解菌的分离筛选及其产酶条件优化[J].安徽农业科学,2011,39(5):2807-2809,2812,4.基金项目
广东省农业科学院科技支撑项目(07-支撑-04) （07-支撑-04）
广东省农业攻关计划项目(2007A0201000043) （2007A0201000043）
国家现代农业产业技术体系建设专项资金资助. （）
