山东医药2011，Vol.51Issue(18)：5-6,2.
川芎嗪、顺铂对人小细胞肺癌NCI-H446细胞凋亡的影响及机制探讨
Effect and mechanism of TMP and DDP on apoptosis of small cell lung cancer cell line NCI-H446
摘要
Abstract
Objective To observe the effect of apoptosis of small cell lung cancer cell line NCI-H446 and explore its mechanism. Methods The NCI-H446 cella in logarithmic phase were divided into control group ( group A), tetramethylpyrazine (TMP) treated group( group B), cisplatin (DDP) treated group(group C) and combined TMP and DDP treated group( group D), four groups were cultured 72 h. The cell apoptosis in each group and the changes of apoptosis-related genes Bcl-2, p53 were observed by flow cytometry and Western blot respectively. Results The apoptosis , Bcl-2 and p53 protein were group A 5.23％ ± 1.01％ ,0.87 ± 0. 003 5, 0. 19 ± 0.002 3; group B 16.37％ ± 1.23％, 0.58 ± 0.004 8,0.31±0.001 4; group C 19.2％ ±1.51％, 0.32±0.003 9, 0.46±0.0025; group D 21.2％ ±1.79％, 0.23 ± 0.001 9, 0.62 ± 0. 002 7. group B, group C and group D corapaired with group A, all P ＜ 0.05, group D compaired with group B or group C, all P ＜ 0.05. Conclusions Combined TMP and DDP can induced apoptosis of NCI-H446 cell.This may have some relationship with the decrease of Bcl-2 protein and the increase of p53 protein.关键词
肺肿瘤/小细胞肺癌/川芎嗪/顺铂/细胞凋亡Key words
lung neoplasm /small cell lung cancer/ tetramethylpyrazine/ cisplatin/ apoptosis分类
医药卫生引用本文复制引用
黄波,郎贤平,王晓东..川芎嗪、顺铂对人小细胞肺癌NCI-H446细胞凋亡的影响及机制探讨[J].山东医药,2011,51(18):5-6,2.基金项目
辽宁省教育厅科研基金资助项目(L2010302,L2010291). （L2010302,L2010291）
