中国农业大学学报2011，Vol.16Issue(3)：117-121,5.
圩猪和定远猪PRLR基因多态性与产仔数的关联分析
Association between polymorphism of prolactin receptor (PRLR) gene and litter size in Wei pigs and Dingyuan pigs
摘要
Abstract
Using modern molecular maker techniques to seek the markers loci which effect the litter size of pig, and provide the foundation for marker assisted selection that cultivating the breed or strain of pigs which have high reproduction rate.This research used PCR-RFLP(Alu Ⅰ restriction enzyme), molecular marker techniques, analyzed the polymorphism of the tenth exon in PRLR gene in 55 Wei Pigs and 63 Dingyuan Pigs.Least-square analysis method was used to study the effects of PRLR gene on litter size.The results showed that, polymorphism exists on the tenth exon in both Wei Pigs and Dingyuan Pigs.The genotype frequency of AA、 AB、 BB were 0.490 9、0.436 4、0.072 7 and 0.507 9、0.396 8、0.095 2 in Wei Pigs and Dingyuan Pigs respectively,where A alleles is the dominant gene,showed 0.7091 and 0.706 3 respectively.The gene frequencies and genotype frequencies of both Wei Pigs and Dingyuan Pigs reached the Hardy-Weinberg equilibrium (P＞0.05).Among three genotypes of the PRLR gene, Wei Pigs and Dingyuan Pigs show no significant difference in the total number born (TNB),the number born alive (NBA) of the first parity and the TNB,NBA of the latter parities (P＞0.05).All results showed the trend of AA＞AB＞BB.Polymorphism in the tenth exon of PRLR gene had certain effect on litter size.However, further study is necessary to get a conclusion that,whether the gene is one of the candidate genes or it linked with the QTL that influence litter size for litter size.关键词
猪/PCR-RFLP/PRLR基因/产仔数/候选基因/标记选择分类
农业科技引用本文复制引用
张陈华,丁月云,王阳,殷宗俊..圩猪和定远猪PRLR基因多态性与产仔数的关联分析[J].中国农业大学学报,2011,16(3):117-121,5.基金项目
安徽省教育厅自然科学基金重点项目(KJ2009A114) （KJ2009A114）
安徽省"十一五"科技攻关项目(07010302136) （07010302136）
教育部科学技术研究重点项目资助(208059) （208059）
