西南国防医药2011，Vol.21Issue(6)：585-587,3.DOI:10.3969/j.issn.1004-0188.2011.06.002
壳聚糖聚乳酸膜的制备和毒性试验研究
Preparation and toxicity test of chitosan-polylactic acid film
摘要
Abstract
Objective To manufacture a biomembrane for the primary repair of colonic fistula, and to evaluate its acute general toxicity and cytotoxicity. Methods After the preparation of polylactic acid film( PAF )and chitosan - polylactic acid film ( CPAF ), acute general toxicity and cytotoxicity examinations were performed with trypanblau cytoactive count and MTT reduction assay according to pharmacopeia for CPAF. Results A method for the preparation of CPAF was successfully established. There were no significant differences in physical performances between PAF and CPAF. No death and paradoxical reaction in mice were found by acute general toxicity test. Trypanblau cytoactive count and MTT reduction assay showed no significant differences between the two biofilms.Conclusion CPAF meets physic requirement of pharmacopeia with no acute general toxicity and cytotoxicity. Its biocompatibility is good according to preliminary assessment.关键词
聚乳酸/壳聚糖/壳聚糖聚乳酸膜/毒性分类
医药卫生引用本文复制引用
陈康,李瑛,谢良宪..壳聚糖聚乳酸膜的制备和毒性试验研究[J].西南国防医药,2011,21(6):585-587,3.基金项目
成都军区"十一五"指令性课题(MB07006) （MB07006）
