中国农业科学2011，Vol.44Issue(7)：1443-1450,8.DOI:10.3864/j.issn.0578-1752.2011.07.017
菊花管状花数量和花心直径的OTL分析
QTL Analysis for Tubular Floret Number and Flower Center Diameter of Chrysanthemum
摘要
Abstract
[Objective]The purpose of this study is to detect main-effect QTL controlling tubular floret number and flower center diameter and provide some reference for molecular marker-assisted breeding of inflorescence traits in chrysanthemum.[Method]In this study, tubular floret number and flower center diameter of chrysanthemum were evaluated in 2008 and 2009, and QTL analysis was undertaken for the two traits based on the two-year phenotypic data in 142 F1 mapping population derived from a cross between chrysanthemum cultivars 'Yuhualuoying' and 'Aoyunhanxiaoh' and their SRAP-based genetic linkage maps.[Result]The two traits of tubular floret number and flower center diameter showed continuous variation and significantly correlated with each other with Pearson correlation coefficient of 0.37 (P＜0.01).The QTL analysis indicated that 2 for tubular floret number and 7 for center floret number QTL were detected based on thc two-year phenotypic data.These QTL scattered on 6 linkage groups, including Y1, Y2 and Y3 of 'Yuhualuoying' map, and A5, Al3 and Al9 of 'Aoyunhanxiao' map, with a LOD value ranging from 2.50 to 4.18 and a contribution ratio to the phenotypic variation of 6.17％-19.95％ for each QTL.TfnE1Y21 and TfnE2Y21 controlling tubular floret number as well as FcdE1Y1 and FcdE2Y1 controlling flower center diameter which were mapped in the same marker interval on the linkage groups of Y21 and Y1 in 2008 and 2009 should be the same QTL and were less affected by environment, respectively.The other QTL detected in either 2008 or 2009 were remarkably affected by environment.[Conclusion]A total of 9 QTL were mapped for tubular floret number and flower center diameter and the major QTL little affected by environment would be of great significance for the future study of molecular marker assisted breeding program in chrysanthemum.关键词
菊花/F1群体/管状花数量/花心直径/QTL分析Key words
chrysanthemum/ F1 population/ tubular floter number/ flower center diameter/ QTL analysis引用本文复制引用
张飞,陈发棣,房伟民,陈素梅,张鲜艳,尹冬梅..菊花管状花数量和花心直径的OTL分析[J].中国农业科学,2011,44(7):1443-1450,8.基金项目
国家自然科学基金项目(30871724) （30871724）
