中国医科大学学报2011，Vol.40Issue(4)：297-300,4.DOI:CNKI:21-1227/R.20110426.1536.023
丝胶对2型糖尿病大鼠海马HO-1表达的影响
Effects of Sericine on HO-1 Expression in Hippocampus of Type 2 Diabetic Rats
摘要
Abstract
Objective To investigate the effects of sericine on heme oxygenase-1 (HO-1) expression in hippocampus of type 2 diabetic rats. Methods 30 male SD rats were randomly divided into 3 groups (n =10 respectively):normal control group, diabetes model group and sericine treatment group. The diabetes model rats were made by continuous intraperitoneal injection of streptozotocin and blood glucose ≥16.7mmol/L was taken as the standard of model-formation. After successfully establishing diabetes rat model,rats in diabetes model group were not treated anymore; rats in sericine treatment group were lavaged with sericine (2.4 g·kg-1·d-1) for 35 days. The morphological changes of nerve cells in hippocampus were observed by HE staining. Western Blotting and RT-PCR were respectively used to detect the expression of HO-1 protein and mRNA in hippocampus. Results In diabetes model rats,the neurons in CA1 region of hippocampus showed obvious pathological changes,the expressions of HO-1 protein and mRNA were obviously higher than normal control rats (P ＜ 0.01 ). Compared with diabetes model rats,the pathological changes of neurons were alleviated significantly (P ＜ 0.01 ),the expressions of HO-1 protein and mRNA in CA1 region of hippocampus in sericine-treated rats decreased significantly (P＜0.05).Conclusion Sericine can alleviated the damages of neurons in hippocampus induced by high expression of HO-1, thus has protective effects on injuries of nervous system during diabetes.关键词
丝胶/糖尿病/海马/血红素加氧酶1分类
医药卫生引用本文复制引用
和亚强,付文亮,马洪伟,宋成军,陈志宏..丝胶对2型糖尿病大鼠海马HO-1表达的影响[J].中国医科大学学报,2011,40(4):297-300,4.基金项目
河北省教育厅项目(2006301),河北省科技厅项目(08276101D-19) （2006301）
