江西农业学报2011，Vol.23Issue(4)：36-38,3.
EMS诱导黄瓜离体变异及分子标记检测
EMS-induced Mutagenesis of Isolated Cucumber and Its Detection by Molecular Markers
摘要
Abstract
Different concentrations of ethyl methane sulfonate (EMS) were used to treat the stem segments of cucumber seedlings in this research. Using the survival rate and bud differentiation rate of the stem segments as criteria, we obtained the half - lethal treatment combination, i.e. treating the stem segment with 4％ EMS for 2 h. RAPD and SRAP analysis showed that DNA variation existed in the regenerated plantlets induced by EMS. The two markers detected eight RAPD variation loci and 5 SRAP variation loci.关键词
EMS/黄瓜/变异/分子标记分类
农业科技引用本文复制引用
胡建斌,刘丹华,王建丽,李雅鸽,马兴帅,张美芳,李建吾..EMS诱导黄瓜离体变异及分子标记检测[J].江西农业学报,2011,23(4):36-38,3.基金项目
河南省科技攻关项目(102102110037) （102102110037）
河南省教育厅自然科学研究计划项目(2009A210010). （2009A210010）
