天然产物研究与开发2011，Vol.23Issue(2)：232-235,285,5.
女贞子对大鼠成骨细胞增殖与分化的影响
Effects of Aqueous Extract of Fructus Ligustri Lucidi on the Proliferation and Differentiation of Osteoblastic UMR-106 cells
摘要
Abstract
To study the effects of Fructus Ligustri Lucidi on the proliferation and differentiation of cultured osteoblasts in vitro, the proliferation and the activity of alkaline phosphatase (ALP) in osteoblastic UMR-106 cells was investigated in the presence of water extract of Fructus Ligustri Lucidi (LWE) with Am-blue method and p-nitrophenyl phosphate disodium salt (PNPP) method respectively. The results showed that LWE promoted the proliferation and differentiation of UMR-106 for 48 h at 100 μg/mL. To study the mechanism of LWE on differentiation,pGL3-5 × ERE-Luc reporter plasmid was transfected into breast cancer cell MCF-7, and the effect of LWE on the expression of lucfferase was investigated. The results indicated that LWE promoted the estrogen receptor responsive element (ERE) driven expression of lucfferase. The promoting effect of LWE on the differentiation of UMR-106 cells could be suppressed by ICI182780,an estrogen receptor antagonist. These results suggested that the mechanism of differentiation promoting effect by LWE may be related to estrogen receptor signaling pathway.关键词
女贞子/UMR-106/增殖/分化Key words
Fructus Ligustri Lucidi/ UMR-106/ proliferation/ differentiation分类
医药卫生引用本文复制引用
王玉梅,陈前锋,杨莉娟,王飞..女贞子对大鼠成骨细胞增殖与分化的影响[J].天然产物研究与开发,2011,23(2):232-235,285,5.基金项目
中国科学院"西部之光"重点资助项目 （）
