安徽农业科学2011，Vol.39Issue(5)：2639-2641,3.
血红铆钉菇液体发酵条件的优化
Optimization of Liqquid Fermentation Conditions of Gomphidius rutilus
高婵娟 1季曼 1杨翔华 1王战勇1
作者信息
- 1. 辽宁石油化工大学环境与生物工程学院,辽宁抚顺,113001
- 折叠
摘要
Abstract
[ Objective] The study aimed to optimize the liquid fermentation conditions of Gomphidius rutilus. [ Method] G. rutilus mycelium was cultured by liquid fermentation, the effects of some influencing factors such as cultivation time and initial pH in medium on the mycelium yield were investigated through the single factor test and the culture conditions suitable to produce the myeelium by liquid fermentation were confirmod through the orthogonal test. [ Result] The single factor test showed that the optimum culture time for G. rutilus mycelium was 6 d at lest, the liquid seed inoculation should be over 10%, the pH value in the medium was as best as 8.5, the liquid volume in flask was 200 mi/S00 mi flask that was beneficial to the growth of mycelium and the optimum rotating speed could be controlled at 180 r/min. The liquid fermentation conditions of G. rutilus optimized by the orthogonal test were as follows: the initial pH value in the medium was 9. 0, the liquid volume in flask was 200 mi/500 ml flask, the liquid seed inoculation was 12.5% , the culture time was 7 d, the culture temperature was 28 ℃, under which, the yield of G. rutilus mycelium could reach to (9. 320 + 0. 151 ) mg/L. [ Conclusion] The study provided the scientific basis for getting high yield of G. rutilus mycelium.关键词
血红铆钉菇/液体发酵/菌丝体Key words
Gomphidius rutilus/ Liquid fermentation/ Mycelium分类
农业科技引用本文复制引用
高婵娟,季曼,杨翔华,王战勇..血红铆钉菇液体发酵条件的优化[J].安徽农业科学,2011,39(5):2639-2641,3.
