分析化学2011，Vol.39Issue(3)：305-311,7.DOI:10.3724/SP.J.1096.2011.00305
雄激素非依赖前列腺癌细胞系代谢组学的初步研究
Metabolomic Profiles in Prostate Cancer Cells Progression
摘要
Abstract
To investigate the biological mechanisms at the basis of androgen resistance, a comparative metabolic approach based on ultra performance liquid chromatography quadrupole-time of flight mass spectrometry (UPLC-Q-TOF-MS) was applied to the human epithelial prostate cancer cell lines LNCaP and its androgen non-dependent counterpart LNCaP-AI/LNCaP-AI+F. Three kinds of cells were quenched by 40％ ethanol-0.8％ sodium chloride solution (-20℃) and 60％ methanol-0. 85％ammonium bicarbonate(-40℃). The cellular metabolism was studied by UPLC-Q-TOF-MS using electron spraying ionization with positive mode to find potential metabolic biomarkers and reveal their physiological changes. After UPLC Q-TOF MS analysis, principal components analysis (PCA),projection to latent structures discriminant Analysis(PLS-DA), orthogonal projection to latent structures discriminant analysis (OPLS-DA) and nonparameter test were done using the Masslynx,SIMCA-P and SPSS for marker selection and identification. Potential biomarker screening was performed according to VIP value, Scatter plot, loading plot and P value. According to the data, most of biomarkers were identified as phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, palmitic amide, steroid hormones, phytosphingosine and SM. Lysophosphatidyl choline,phosphatidylcholine with lower unsaturated acyl chains (n＞10) and taurocholat higher time-dependent increasing trends, while phosphatidylcholine with lower unsaturated acyl chains (n＜ 4) and Palmitic amide,steroid hormones, Phytosphingosine show decreasing trends. The results indicate that there are differential metabolic biomarkers between androgen-dependent and androgen-independent cells of human prostate cancer especially in lipids.关键词
雄激素非依赖性的前列腺癌/代谢组学/代谢标志物/超高效液相色谱-四极杆-飞行时间质谱Key words
Non-dependent prostate cancer cell/ Metabolomics/ Metabolic biomarker/ Ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry引用本文复制引用
刘永霞,朱小丽,陈玉华,郭飞,沈默,陈占国,徐威,陈德莹,陶志华..雄激素非依赖前列腺癌细胞系代谢组学的初步研究[J].分析化学,2011,39(3):305-311,7.基金项目
本文系国家自然科学基金(No.30872421/C160102)及浙江省科技厅项目基金 (No.2009F70015)资助 （No.30872421/C160102）
