分析化学2011，Vol.39Issue(3)：312-316,5.DOI:10.3724/SP.J.1096.2011.00312
新型纳米纤维固相萃取柱制备及其用于组织样品中柔红霉素测定研究
Preparation of Novel Packed-Nanofibers Solid Phase Extraction Column and Its Application to the Determination of Daunorubicin in Rat Tissues
摘要
Abstract
A novel packed-nanofibers solid-phase extraction column has been investigated for purification and enrichment of daunorubicin in plasma and different tissues of rat (heart, liver, spleen, lung, kidney). Meanwhile, an HPLC method with fluorescence detection for determination of biological samples has been developed. 10％ (V/V) HCIO4 was used to extract daunorubicin from biological samples. After homogenizing for lmin and centrifuging for 10 min at 12000 r/min,the supernatant was purified and preconcentrated by the column, then only 50 μl of eluant (methanol-acetic acid (99:1, V/V)) was required to desorb the target compounds. The mobile phase was methanol-water-acetic acid (60: 40:0.25, V/V, pH 5.5), the flow rate was 1.0 mL/min. Results indicated that the linear range of claunorubicin was 0. 02-20 μg/g in the tissues samples and 0. 01 - 1.00 mg/L in the plasma samples. The recoveries were in the range of 82. 7％ - 112. 3％. RSDs.for intra-day and inter-day were 2. 0％-9.9％ and 5.2％-11.9％ respectively. The method is rapid and sensitive, which can be applied to determine daunorubicin in biological samples.关键词
柔红霉素/纳米纤维/高效液相色谱Key words
Daunorubicin/ Nanofiber/ High performance liquid chromatography引用本文复制引用
曲子健,康学军,吴玮玮,陈宝安,顾忠泽..新型纳米纤维固相萃取柱制备及其用于组织样品中柔红霉素测定研究[J].分析化学,2011,39(3):312-316,5.基金项目
本文系国家"973"基金项目资助(No.2007CB936300),国家自然基金项目资助(No.30872970),苏州市应用基础研究项目资助(Nos.SYJG0912,SYN201006)和江苏省工业支撑项目(No.BE2010088)资助 （No.2007CB936300）
