广东药学院学报2011，Vol.27Issue(2)：124-129,6.DOI:10.3969/j.issn.1006-8783.2011.02.004
新型铋(Ⅲ)卟啉配合物的合成、表征及其与小牛胸腺DNA相互作用的初步研究
Synthesis, characterization and DNA binding properties of a new Bi (m) porphyrin complex
摘要
Abstract
Objective To synthesize a new bismuth porphyrin and investigate its interaction with calf thymus DNA ( CT-DNA ). Methods A new bismuth porphyrin Bi [ MPyTMOPP ] NO3 was synthesized and characterized by elemental analysis,1H-NMR, MS spectra (ESI-MS), IR and UV-Vis spectroscopy. The interaction of Bi[ MPyTMOPP] NO3 and CT-DNA was investigated by UV-Vis spectroscopy,fluorescence spectroscopy, circular dichroism (CD) spectroscopy, thermal denaturalization and viscosity experiments.Results The ESI-MS spectra of Bi [ MPyTMOPP ] NO3 had a ionic peak contributed to ( M + H) + at 912.47;In the Sorer band of UV-Vis spectra, a peak with minor hypochromism and bathochromism (reaching 13.7％ and 3 nm) was observed,and the intrinsic binding constant was determined to be 8.6 × 103 L/mol. A positively induced CD band appeared in the Soret band of Bi[ MPyTMOPP] NO3 with DNA.The melting temperature of CT-DNA increased by 2.1 ℃ ,and the relative viscosity of CT-DNA didn't vary with the concentration of Bi[ MPyTMOPP] NO3. Conclusion The results suggest that Bi[ MPyTMOPP] NO3 may interact with CT-DNA by outside groove binding.关键词
铋卟啉配合物/小牛胸腺DNA/结构表征/相互作用Key words
bismuth porphyrin complex/ calf thymus DNA/ structure indentification/ interaction分类
药学引用本文复制引用
尹鹏,梅文杰,王伟章,王淇,孙福强,王成蹊,贺丽敏..新型铋(Ⅲ)卟啉配合物的合成、表征及其与小牛胸腺DNA相互作用的初步研究[J].广东药学院学报,2011,27(2):124-129,6.基金项目
广东省自然科学基金博士启动基金(04300624) （04300624）
广东省科技计划项目(2007B031513004) （2007B031513004）
广东药学院中青年骨干教师培养基金资助 （）
