广东药学院学报2011，Vol.27Issue(2)：147-150,4.DOI:10.3969/j.issn.1006-8783.2011.02.010
柿蒂药材的高效液相色谱指纹图谱研究
Study on chromatographic fingerprint in Persimmon Calyx by HPLC
周本宏 1沈恒 2魏嫣 2张琛霞 2邱振鹏 2刘刚2
作者信息
- 1. 武汉大学人民医院药学部,湖北,武汉,430060
- 2. 武汉大学药学院,湖北,武汉,430072
- 折叠
摘要
Abstract
Objective To establish the chromatographic fingerprint of Persimmon Calyx by HPLC. Methods The HPLC method was performed on a Agela venusil MP-C18 (250 mm × 4.6 mm,5 μm), and the mobile phase was methanol-0.5％ acetic acid with gradient elution at the flow rate was 0.8 mL/min. The detection wavelength was 264 nm, and the temperature was at 20 ℃, and 20 μL was injected. Results The HPLC fingerprint of Persimmon Calyx was established preliminarily, in which 15 characteristic peaks were obtained and the similarity among 10 batches of Persimmon Calyx from different areas was above 80％. Conclusion The HPLC fingerprint method is stable and repeatable,which can be used to evaluate the quality of Persimmon Calyx.关键词
柿蒂/指纹图谱/高效液相色谱法Key words
Persimmon Calyx/ fingerprint/ HPLC分类
医药卫生引用本文复制引用
周本宏,沈恒,魏嫣,张琛霞,邱振鹏,刘刚..柿蒂药材的高效液相色谱指纹图谱研究[J].广东药学院学报,2011,27(2):147-150,4.
