广东药学院学报2011，Vol.27Issue(2)：170-173,4.DOI:10.3969/j.issn.1006-8783.2011.02.017
复方斑蝥含药血清抑制人肝癌Bel-7402细胞增殖的机制研究
Inhibitory effect of compound mylabris on the proliferation of human hepatocarcinoma cell line Bel-7402 and its molecular mechanism
摘要
Abstract
Objective To investigate the molecular mechanism underlying the inhibitory effect of compound mylabris on the proliferation of human hepatocarcinoma cell line Bel-7402. Methods The compound mylabris serum was prepared based on serum pharmacology and was used to treat human hepatocarcinoma cell line Bel-7402. MTr assay was used to evaluate the inhibitory effect of compound mylabris on the proliferation of human hepatocarcinoma cell; RT-polymerase chain reaction (RT-PCR) was used to evaluate c-Myc and p53 mRNA expressions in human Hepatocarcinoma cell Bel-7402. Results Compound mylabris serum can remarkably inhibit the proliferation of human hepatocarcinoma cell line Be1-7402 in which gene c-Myc was down-regulated, while p53 was up-regulated. Conclusion Compound mylabris possibly affects the expressions of genes related to the proliferation of human hepatocarcinoma cell line Bel7402. It is indicated that compound myabris can be used as a prototype for compound traditional Chinese medicine against liver cancer.关键词
复方斑蝥/人肝癌细胞Bel-7402/p53/c-Myc/细胞增殖Key words
compound mylabris/ human hepatocarcinoma cell line Bel-7402/ p53/ c-Myc/ cell proliferation分类
中医学引用本文复制引用
姜新根,肖兰凤,黄树林,刘顺会..复方斑蝥含药血清抑制人肝癌Bel-7402细胞增殖的机制研究[J].广东药学院学报,2011,27(2):170-173,4.基金项目
国家自然科学基金(30572124) （30572124）
