分析化学2011，Vol.39Issue(3)：409-413,5.DOI:10.3724/SP.J.1096.2011.00409
新型壳聚糖荧光纳米粒的制备及pH敏感性能研究
Synthesis and Properties of New pH Sensitive Chitosan Fluorescent Nanoparticles
摘要
Abstract
A new naphthalimide kind of carboxyl-containing fluorophore was synthesized, and then the fluorescent polymer naphthalimide-modified carboxymethyl chitosan(CMCS3N) was developed by the way of chemical bonding of the synthesized naphthalimide fluorophore into carboxymethyl chitosan (CMCS), a water-soluble polymer with good biocompatibility. The structures and morphology of CMCS3N have been characterized by the methods of infrared spectroscopy, ultraviolet-visible spectroscopy and transmission electron microscopy. The results showed that the fluorescent polymer has nanostructure with diameters of about 40 nm. The fluorescence microscopy images illustrated CMCS3N could give yellow-green fluorescence under UV-light. The excitation and emission wavelengths of the nanoparticles have been obtained by fluorescent spectroscopy with 409 nm and 534 nm,respectively. The fluorescence of CMCS3N was sensitive to pH in the range from pH 5 to 8 and 1. O×10-5 mol/L of common metal cations had no obvious effect on the fluorescence intensity. The results indicated CMCS3N could be used as pH sensitive fluorescent probes in vivo imaging.关键词
萘酰亚胺/羧甲基壳聚糖/荧光纳米粒/pH敏感Key words
Naphthalimide/ Carboxymethyl chitosan/ Fluorescent nanoparticles/ pH sensitive引用本文复制引用
余慧,梁淑彩,钟海迪,付婷,鄢国平..新型壳聚糖荧光纳米粒的制备及pH敏感性能研究[J].分析化学,2011,39(3):409-413,5.基金项目
本文系国家自然科学青年基金(No.20905059)及中央高校基本科研业务费专项资金(No.3082014)资助项目 （No.20905059）
