大连工业大学学报2011，Vol.30Issue(3)：173-176,4.
绞股蓝总皂苷中单体皂苷的分离纯化
Purification of gypenoside hydrolysate
摘要
Abstract
The total gypenoside was separated and purified by silica gel column. Chloroform and methanol were used as mobile phase in gradient elution, the sample was determined by TLC. 7.87 g total saponin was obtained from 5000 g Gynostemma pentaphyllum by the process of extracted with alcohol, defatted with ligarine, leached with normal butanol, desugared with AB-8 macro porous resin, decolored with D-296 anion ion exchange resin. Three kinds of gypenoside monomer were obtained from 5 g total gypenoside by the proportion in 9.5∶0.5 and 9∶1 chloroform and methanol as mobile phase. The yields were 2.6％, 3.0％, 5.2％ and the purity was up to 88. 73％, 93.11％,78.54％ respectively. The result indicated that the silica gel column is a good separation function to gypenoside.关键词
绞股蓝皂苷/硅胶柱层析/高效液相色谱Key words
gypenoside/ silica gel column/ HPLC分类
轻工纺织引用本文复制引用
孔玉琼,金凤燮,鱼红闪..绞股蓝总皂苷中单体皂苷的分离纯化[J].大连工业大学学报,2011,30(3):173-176,4.基金项目
国家自然科学基金资助项目(30371744) （30371744）
辽宁省科学技术基金资助项目(20042132) （20042132）
辽宁省教育厅创新团队项目(2009T007). （2009T007）
