水产学报2011，Vol.35Issue(5)：719-728,10.DOI:10.3724/SP.J.1231.2011.17295
盐度变化对斜带石斑鱼生理生化因子的影响
The effect of salinity change on physiology and biochemistry of Epinephelus coioides
摘要
Abstract
The objectives of this work were to study the effects of salinity change on the physiological chemical factors of Epinephelus coioides juvenile [body weight: (19.59 ± 0.25) g]by a biochemical analysis.The gill Na +/K + -ATPase, plasma glucose, aspartate carbamyl transferase ( AST ) , the activity of SOD and lysozyme of Epinephelus coioides juvenile were assayed after being treated with salinity[(4,14,24 and 34 as control )]in 1,3,6 and 9 d At the same time, the gill chloride cells were observed using an H-E staining.The results showed that Na+/K+-ATPase activity in all groups showed similar tendency and the concentration reached the maximum level on 3rd day, then decreased and keep it constant on 6th day.The number of gill chloride cells did not show significant difference in salinity 24, but decreased slightly in salinity 14 during the experiment period.The changes in number, nucleus size and color of gill chloride cells in salinity 4 were observed on 6th day.The serum glucose level in all groups showed similar tendency and the concentration reached the maximum level on 6th day, then decreased.The serum AST level showed similar tendency both in salinity 24 and in salinity 4 and the concentration reached the maximum level on 9th day.However,the serum AST level continuously decreased in salinity 14 during the experiment period.The hepatic SOD activity in all test groups showed a tendency of decreasing at the beginning and increasing later,and reached the minimum level on 3rd day.There were significant differences in the hepatic SOD activity among all test groups ( P ＜ 0.05 ).The serum lysozyme activity in all test groups showed a tendency of increasing at the beginning and reached the maximum level on 6th day, then decreased and gradually stable.There were significant differences in serum lysozyme activity among all test groups ( P ＜ 0.05 ).When the salinity gradually decreased from 34 to 24,14 and 4, these physiological chemical indicators showed similar tendency in all test groups, suggesting that E.coioides is typical of broad salinity fish and possesses good adaptability to low salinity.The results showed that E.coioides juveniles could adapt to the water body of salinity 4 under salinity domestication.关键词
斜带石斑鱼/盐度胁迫/Na+/K+-ATP酶/氯细胞/血糖/天门冬氨酸氨基转移酶/超氧化物歧化酶/溶菌酶Key words
Epinephelus coioides/ salinity stress/ Na +/K + -ATPase/ chloride cells/ plasma glucose/aspartate carbamyl transferase/ superoxide dismutase/ lysozyme分类
生物科学引用本文复制引用
余德光,杨宇晴,王海英,谢骏,郁二蒙,王广军,龚望宝..盐度变化对斜带石斑鱼生理生化因子的影响[J].水产学报,2011,35(5):719-728,10.基金项目
广东省科技计划项目(2008A020800012) （2008A020800012）
现代渔业产业技术体系建设项目(nycytx49) （nycytx49）
珠海市科技项目(c20082031) （c20082031）
广东省科技计划项目(2009B020202015) （2009B020202015）
国家海洋公益性行业科研专项(200805047) （200805047）
