色谱2011，Vol.29Issue(2)：120-125,6.DOI:10.3724/SP.J.1123.2011.00120
分散固相萃取-超高效液相色谱-串联质谱法测定鸡肝中磺胺类、喹诺酮类和苯并咪唑类药物及其代谢物的残留量
Determination of sulfonamides,quinolones, benzimidazoles and the metabolites of benzimidazoles in chicken livers by dispersive solid-phase extraction and ultra performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A method for the determination of 12 sulfonamides, 19 quinolones and 8 benzimidazoles and the metabolites of benzimidazoles in chicken livers by quick , easy , cheap , effective ,rugged and safe ( QuEChERS ) extraction and ultra performance liquid chromatography-tandem mass spectrometry ( UPLC-MS/MS ) has been developed. The samples were extracted with 1％acetic acid-acetonitrile solution, cleaned up with amine ( NH2 ) sorbent and defatted with nhexane. The identification and quantification were achieved by using electrospray ionization in positive ion mode ( ESI+ ) with multiple reaction monitoring ( MRM ). The matrix-matched internal standard calibration curves were used for quantitative determination. The linear range was from 5 to 100 μg/kg. The average recoveries and relative standard deviations were 72％ -121％ and 1.5％ -23.4％ respectively in the spiked range of 10 -50 μg/kg. The limits of detection were 5 μg/kg and the limits of quantification were 10 μg/kg for the 39 drugs. The method is simple , rapid, sensitive and accurate. It is suitable for the quantitative determination and confirmation of 12 sulfonamides, 19 quinolones, 8 benzimidazoles and the metabolites of benzimidazoles.关键词
分散固相萃取/超高效液相色谱-串联质谱法/磺胺/喹诺酮/苯并咪唑/鸡肝Key words
dispersive solid-phase extraction/ ultra performance liquid chromatography-tandem mass spectrometry ( UPLC-MS/MS )/ sulifonamides/ benzimidazoles/ quinolones/ chicken livers分类
化学化工引用本文复制引用
李锋格,苏敏,李晓岩,张洪霞,姚伟琴,窦辉,张万权..分散固相萃取-超高效液相色谱-串联质谱法测定鸡肝中磺胺类、喹诺酮类和苯并咪唑类药物及其代谢物的残留量[J].色谱,2011,29(2):120-125,6.基金项目
国家质量监督检验检疫总局科研项目(No.2007IK174). （No.2007IK174）
