色谱2011，Vol.29Issue(2)：126-130,5.DOI:10.3724/SP.J.1123.2011.00126
超高效液相色谱-串联质谱法测定人尿液中全氟有机化合物
Determination of perfluorinated compounds in human urine by ultra high performance liquid chromatography tandem mass spectrometry
摘要
Abstract
A method for the analysis of 12 perfluorinated compounds ( PFCs ) in human urine by ultra high performance liquid chromatography-tandem mass spectrometry ( UPLC-MS/MS was developed and validated. One mL 2％ formic acid in methanol was added into the urine.After ultrasonication and centrifugation , the samples were purified by a solid phase extraction column and examined by UPLC-MS/MS. The target compounds were quantified by stable isotope dilution technique. The linear range was 0.05 -50 μg/L for the 12 PFCs and the correlation coefficient ≥ 0.992. The limits of detection of 12 PFCs were in the range of 0.44 - 3.47 ng/L. The matrix recoveries of the method for the 12 PFCs in three spiked levels ( 20, 100, 500 ng/L ) ranged from 80.3％ to 116.2％. The relative standard deviations ( RSDs, n = 5 ) were between 5.5％ and 13.8％. The sensitive and accurate method was successfully applied to the analysis of PFCs in human urine.关键词
超高效液相色谱-串联质谱/全氟有机化合物/尿液Key words
ultra high performance liquid chromatography-tandem mass spectrometry ( UPLCMS/MS ) / perfluorinated compounds / urine分类
化学化工引用本文复制引用
郭斐斐,王雨昕,李敬光,张加玲,赵云峰,吴永宁..超高效液相色谱-串联质谱法测定人尿液中全氟有机化合物[J].色谱,2011,29(2):126-130,5.基金项目
国家自然科学基金项目(No.21077089.No.20837003)、卫生行业科研专项(No.200902009)和国家科技支撑计划项目(No.2007BAC27802). （No.21077089.No.20837003）
