水生生物学报2011，Vol.35Issue(3)：436-442,7.DOI:10.3724/SP.J.1035.2011.00436
RNA解旋酶基因crhR对于集胞藻PCC6803低温适应的作用
THE ROLE OF CRHR, THE RNA HELICASE GENE,IN COLD ACCLIMATION OFSYNECHOCYTIS SP.PCC 6803
摘要
Abstract
Cold acclimation of cyanobacteria involves the regulation of many genes, such as crhR that encodes RNA helicase.In this study, the transcription ofcrhR was examined in Synechocystis sp. PCC 6803 transferred from 30℃ to 15℃, and its transient expression was found within 2h. Inactivation of this gene in the cyanobacterium led to loss of growth at 15℃, great reduction of photosynthetic and respiratory activities, and inability to eliminate lipid peroxides. Overexpression of crhR from PrbcL in the crhR mutant fully complemented the mutation, and the complemented strain showed slightly improved growth at 15℃ compared to the wild type. In the wild type, temperature downshift induced the up-regulation of fatty acid desaturase genes desA, desB and desD and the increase of unsaturation degree of fatty acids. In the crhR mutant, the induction of desB by temperature downshift was significantly reduced, and the contents of polyunsaturated fatty acids were not increased under the same conditions. It is proposed that crhR may directly affect protein synthesis in the cyanobacterium under low-temperature stress, or indirectly, exert its effects on enzyme activities by regulating the expression of chaperone genes.关键词
crhR/低温/集胞藻PCC6803分类
生物科学引用本文复制引用
李琼,徐旭东..RNA解旋酶基因crhR对于集胞藻PCC6803低温适应的作用[J].水生生物学报,2011,35(3):436-442,7.基金项目
国家自然科学基金项目(30825003) （30825003）
973项目课题2008CB418001资助 （）
