动物营养学报2011，Vol.23Issue(5)：842-847,6.DOI:10.3969/j.issn.1006-267x.2011.05.019
N-乙酰半胱氨酸对脂多糖刺激仔猪空肠黏膜抗氧化能力的影响
Effects of Dietary N-acetylcysteine on the Antioxidant Capacity of Jejunal Mucosa of Piglets Challenged with Lipopolysaccharide
摘要
Abstract
The study was conducted to investigate the effects of N-acetylcystine (NAC) on the antioxidant capacity of jejunal mucosa of piglets chronically challenged with lipopolysaccharide (LPS). Eighteen healthy piglets [ average body weight, ( 11.58 ± 0.26 ) kg ] were randomly allocated into 3 groups ( control group, LPS group and NAC group). Each group included 6 replicates with one piglet per replicate. The control group and LPS group were fed the basal diet, and NAC group was fed the basal diet supplemented with 0.05 ％ NAC. On d 10, 13 and 20, piglets in the LPS and NAC groups were injected intraperitoneally with LPS at 100 μg/kg BW,whereas piglets in the control group were injected intraperitoneally with normal sterile saline at the same volume.On d 21, all pigs were sacrificed to obtain jejunal mucosa for analysis. The results showed as follows: 1) LPS challenge reduced the activities of superoxide dismutase ( SOD, P ＜0. 01 ) and catalase ( CAT, P ＜0. 01 ), and increased the activities of nitric oxide synthase ( NOS, P ＜ 0. 05) and inducible nitric oxide synthase ( iNOS,P ＜0. 01 ) in jejunal mucosa of the piglets; the supplementation of 0. 05％ NAC in the diet increased CAT activity ( P ＜0. 01 ), and reduced iNOS activity ( P ＜0. 01 ) in jejunal mucosa of the piglets challenged with LPS. 2 )LPS challenge increased the contents of malondialdehyde ( MDA, P ＜ 0. 05 ), hydrogen peroxide ( H2 O2, P ＜0. 05) and superoxide anion (O2-, P ＜0. 01 ), and glutathione oxidized/glutathione ( GSSG/GSH, P ＜0. 01 )ratio in jejunal mucosa of the piglets; the supplementation of 0. 05％ NAC in the diet reduced the contents of MDA ( P ＜ 0. 05), H2 O2 ( P ＜ 0. 05) and O2- ( P ＜ 0. 01 ), and GSSG/GSH ratio ( P ＜ 0. 01 ) in jejunal mucosa of the piglets challenged with LPS. In conclusion, the supplementation of 0. 05 ％ NAC in the diet can effectively alleviate the oxidative stress in intestinal mucosa of the piglets induced by LPS challenge. [ Chinese Journal of Animal Nutrition, 2011, 23(5):842-847]关键词
N-乙酰半胱氨酸/脂多糖/仔猪/空肠黏膜/抗氧化能力Key words
N-acetylcysteine/ LPS/ piglets/ jejunal mucosa/ antioxidant capacity分类
农业科技引用本文复制引用
张伟,杨震国,侯永清,丁斌鹰,朱惠玲,刘玉兰,王蕾..N-乙酰半胱氨酸对脂多糖刺激仔猪空肠黏膜抗氧化能力的影响[J].动物营养学报,2011,23(5):842-847,6.基金项目
武汉市学科带头人计划项目(200951830554)、湖北省自然科学基金计划创新群体项目(2007ABC009) （200951830554）
