医药导报2011，Vol.30Issue(7)：839-842,4.DOI:10.3870/yydb.2011.07.002
美沙拉嗪缓释剂对2,4,6-三硝基苯磺酸诱导大鼠溃疡性结肠炎的治疗作用
Effect of Mesalazine Retarder on Experimental Ulcerative Colitis by Trinitrobenzene Sulfonic Acid in Rats
摘要
Abstract
Objective To investigate the effects of Mesalazine retarder on the expression of tumor necrosis factor-α ( TNF-α) ,interleukin-1β ( IL-1 β ) and interleukin-6 ( IL-6 ) in trinitrobenzene sulfonic acid ( TNBS ) -induced ulcerative colitis (UC) in rats and explore its anti-inflammatory mechanism. Methods UC was induced in rats with TNBS and ethanol. The rats were randomly divided into three groups : normal control( group C) , model control( group T) , mesalazine retarder treatment group(100 mg · kg-1 · d-1 ) ( group M) and 5-ASA treatment group ( 100 mg · kg-1 · d-1 ) ( group A). One day after UC was induced, the rats in the treatment group were intragastric administered mesalazine retarder or 5-ASA every 12 hours for seven days, while the other three groups were taken an intragastric treatment of distilled water. The disease index( DAI) , body weight and scores of pathological changes ( HS) of rats were calculated. The activity of myeloperoxidase ( MPO) was measured by biochemical method, and expressions of TNF-α、IL-1β and IL-6 mRNA were measured by Real-time PCR, respectively.Results Compared with the normal group,the model rats showed significantly increased DAI, HS score and MPO activity in the colon tissues( P＜0.05 ) , and up-regulated expression of TNF-α、IL-1β and IL-6 mRNA in the colon mucosa (P＜0. 05) as well.Compared with the model and 5-ASA treatment group, mesalazine retarder resulted in a significant reduction in DAI, HS score and MPO activity(P＜0. 05 ) ,and lower expression of TNF-α、IL-1β and IL-6 mRNA ( P＜0. 05 ) in the colon mucosa. Compared with the model group,5-ASA treatment group led to a reduction in DAI, HS score and MPO activity, and decreased expression of TNF-α、IL-1β and IL-6 mRNA in the colon mucosa, but no statistic significance was found. Conclusion Mesalazine retarder has protective effect against rat ulcerative colitis, the mechanism of which probably links to reduction of TNF-α,IL-1β and IL-6 expression and neutrophil infiltration.关键词
美沙拉嗪缓释剂/结肠炎,溃疡性/2,4,6-三硝基苯磺酸/肿瘤坏死因子α/白细胞介素Key words
Mesalazine retarder/ Colitis, ulcerative/ Trinitrobenzene sulfonic acid/ Tumor necrosis factor-α/分类
医药卫生引用本文复制引用
李进,邓豫,李兆明,曹小年,李小兰,陶德定,胡俊波,王晶..美沙拉嗪缓释剂对2,4,6-三硝基苯磺酸诱导大鼠溃疡性结肠炎的治疗作用[J].医药导报,2011,30(7):839-842,4.基金项目
国家自然科学基金资助项目(基金编号:30872472,30973496,30800569) （基金编号:30872472,30973496,30800569）
湖北省自然科学基金资助项目(基金编号:2008CDB174,2009CDB239) （基金编号:2008CDB174,2009CDB239）
