医药导报2011，Vol.30Issue(7)：843-845,3.DOI:10.3870/yydb.2011.07.003
TAT-N24穿膜融合多肽对前列腺癌细胞增殖的影响
Effects of Cell-Permeable TAT-N24 Fusion Peptide on Prostate Cancer Cell Proliferation
摘要
Abstract
Objective To investigate the anti-proliferation effects of cell-permeable TAT-N24 fusion peptide on prostate cancer cells. Methods The human prostate cancer cell line PC3 was treated with TAT-N24 , cell cycle distribution was analyzed by flow cytometry,DNA synthesis of which was measured by BrdU incorporation assay, and the expression of phosphorylated AKT was examined by Western blot analysis. Results ① G0/G1 phase arrest induced by TAT-N24 was observed. The cell cycle distribution was that : in the blank group G0/G1 phase as (56. 9±6. 1)％ , S phase (27. 0±2. 3)％ , G2/M phase ( 16. 1±1. 3)％ ;G0/G1 phase (57.9±4.8)％ , S phase (24. 2±3. 1) ％ , G2/M phase (27. 8±1.4)％ in the control peptide group; G0/G1 phase ( 68. 6±5. 4) ％ ( P＜0. 05 ) , S phase ( 19. 4± 1. 5) ％ ( P＜0. 05 ) , G2/M phase ( 12. 3±1. 4) ％ in TAT-N24 group, respectively. ②The BrdU positive cells in the hlank, control peptide and TAT-N24 groups was ( 37. 9±3. 2) ％ , (36. 2±4. 1) ％ , (21. 5±2. 4) ％ ( P＜0. 05 ) , respectively; ③It was revealed that TAT-N24 had no significant effects on AKT phosphorylation Conclusion The cell-permeable TAT-N24 fusion peptide effectively blocks cells processing into G0/G1 phase and inhibiting DNA synthesis of PC3 cell line. TAT-N24 may become an effective molecular targeted gene medicine for cancer therapy.关键词
TAT-N24穿膜融合多肽/前列腺癌细胞/分子靶向治疗Key words
Cell-permeable TAT-N24 fusion peptide / Prostate cancer cell line/ Molecular targeting therapy分类
医药卫生引用本文复制引用
邓豫,王桂华,左学良,董硕,金源,李维娜,龚建平,胡俊波..TAT-N24穿膜融合多肽对前列腺癌细胞增殖的影响[J].医药导报,2011,30(7):843-845,3.基金项目
国家重点基础研究发展计划(973计划)项目(基金编号:2009CB521802),国家自然科学基金资助项目(基金编号:30872472,30973496,30800569),湖北省自然科学基金资助项目(基金编号:2008CDB174,2009CDB239) （973计划）
