医药导报2011，Vol.30Issue(7)：846-848,3.DOI:10.3870/yydb.2011.07.004
西红花酸对大鼠脑缺血-再灌注氧自由基及一氧化氮的影响
Effect of Crocetin on Free Radicals and Nitrogen Monoxidum During Reperfusion after Cerebral Ischemia in Rats
摘要
Abstract
Objective To investigate the protective effect and mechanism of crocetin on focal cerebral ischemia-reperfusion injury. Methods 120 SD rats were divided into six groups, named as sham operation (sham group) , model control of cerebral ischemia-reperfusion injury (model group) , nimodipine positive control(Nim group) , high dose of crocetin group (40 mg · kg-1) , moderate dose of crocetin group (20 mg·kg-1 ) , low dose of crocetin group (10mg · kg-1 ). The sham group and model group were given with the same dose of normal saline. The rats were treated once in each group in the morning by reperfusion for a week. The model of neurological deficits was made by 2 h of middle cerebral artery occlusion followed with 22 h reperfusion. To observe the nerve functional defect score, cerebral infarction, and check the content of malondialdehyde (MDA) , nitrogen monoxidum oxide (NO) and activity of superoxide dismutase(SOD) in ischemic brain tissues. Results Crocetin improved cerebral infarction and nerve function, enhanced SOD activity, reduced MDA and NO content in a dose-dependent manner. Conclusion Crocetin can protect focal cerebral ischemia-reperfusion injury, the mechanism of which may be related to SOD activity enhancement and reduction of MDA and NO levels.关键词
西红花酸/脑缺血-再灌注,局灶性/自由基/一氧化氮Key words
Crocetin/ Ischemia-reperfusion injury, focal cerebral/ Free radicals/ Nitrogen monoxidum分类
医药卫生引用本文复制引用
谭安雄,朱耀斌,王玉银..西红花酸对大鼠脑缺血-再灌注氧自由基及一氧化氮的影响[J].医药导报,2011,30(7):846-848,3.基金项目
邵阳市科技计划项目(基金编号:N0962) （基金编号:N0962）
