医药导报2011，Vol.30Issue(7)：852-855,4.DOI:10.3870/yydb.2011.07.006
3-氨基苯甲酰胺联合顺铂抑制骨肉瘤细胞生长的作用
Suppression of Osteosarcoma Cells Growth by 3-aminobenzamide Combined with Cisplatin
郑亚东 1徐西强 1彭飞 1虞冀哲 1吴华1
作者信息
- 1. 华中科技大学同济医学院附属同济医院骨科,武汉,430030
- 折叠
摘要
Abstract
Objective To investigate the anticancer effects of cisplatin co-treated with PARP-1 inhibitor 3-aminobenzamide (3-AB) on human osteosarcoma cells. Methods Cell count kit-8 was used to analyze cell proliferation. In CCK-8 assay, U2OS cells were divided into two groups, one group was treated with cisplatin, and the other was treated with cisplatin plus 3-AB. Cell apoptosis and cell cycle were assessed by flow cytometric detection. Results The IC50 of U2OS cells by cisplatin plus 3-AB was lower than that by cisplatin alone, and the sensitized ratio was 1. 76. It was showed that the apoptosis rate was increased in cells treated with 3-AB and cisplatin compared with that treated with two agents alone. And the combination treatment induced a more sustained S phase arrest. Conclusion PARP-1 inhibitor 3-AB enhances the anti-proliferation and apoptosis-inducing effects of cisplatin in human osteosarcoma cells.关键词
3-氨基苯甲酰胺/顺铂/聚腺苷酸二磷酸核糖转移酶-1抑制药/骨肉瘤Key words
3-aminobenzamide/Cisplatin/ PARP-1 inhibitor/ Osteosarcoma分类
医药卫生引用本文复制引用
郑亚东,徐西强,彭飞,虞冀哲,吴华..3-氨基苯甲酰胺联合顺铂抑制骨肉瘤细胞生长的作用[J].医药导报,2011,30(7):852-855,4.
