安徽农业科学2011，Vol.39Issue(12)：7279-7282,4.
利用体细胞核移植技术生产表达红色荧光蛋白的猪转基因克隆胚胎研究
Production of Transgenic Clone Pig Embryos Expressing Red Fluorescent Protein by Somatic Nuclear Transfer Techmology
摘要
Abstract
[ Objective ] The aim was to lay a foundation for producing transgenic clone pig. [ Method ] We use pig fetus fibroblaste with red fluorescent protein (RFP) that transfect with retrovirns for donor of nuclear transfer, utilize somatic cell cloning technology to research the developmentin vifro of cloning embry expressing red fluorescent protein. [ Result ] The results indicated that fusion rate of RFP transgene cell and the control are 83.87％ and 80.56％ respectively, without significant difference ( P ＞ 0.05 ); b lastula rate in vitro of RFP transgene cell reconstructod embryo is 8.67％ compare to the control (6.56％) without significant difference (P ＞0.05 ); RFP transgene cell reconstructed embryo transfer to fifteen surrogacy and none conception. [ Conclusion] Use of red fluorescent protein transgene cell for donor cells can successfully clone transgenic embryo, obtain transgenic blastula.关键词
转基因/红色荧光蛋白/核移植/猪Key words
Transgene/ Red fluorescent protein/ Nuclear transfer/ Pig分类
农业科技引用本文复制引用
康锦丹,尹熙俊,赵明辉,梁爽,刘希,李文学,崔成哲..利用体细胞核移植技术生产表达红色荧光蛋白的猪转基因克隆胚胎研究[J].安徽农业科学,2011,39(12):7279-7282,4.基金项目
国家自然科学基金项目(30960175) （30960175）
农业部"转基因生物新品种培育重大专项"项目(2009ZX08006-002B). （2009ZX08006-002B）
