工业微生物2011，Vol.41Issue(3)：17-20,4.DOI:10.3969/j.issn.1001-6678.2011.03.004
葡萄藻冷冻保藏的研究
Study on cryopreservation of Botryococcus braunii
摘要
Abstract
In order to provide stable and reliable microalgal source for the biofuel production from large scale micmalgae culture , cryopreservation of Botryococcus braunii was investgated and a fast and effective method of cryopreservation was estabjished . Five cryoprotectants (din ethyl sulphoxide , DM SO ; methanol, M eO H ; ethylene glycol, EG ; propylene glycol, PG ; and glycerol, G ly ) at different concentrations were evaluated to determ ine their toxicity and post-thaw viability for B . braunii. The results showed that the optinal post-thaw viability of 98 .9％ was achieved at 6％ of M eO H . Different cooling procedures were also studied and it indicated that the program med cooling was essenrial for cryopreservation of B . braunii. Culture age of B . braunii affected its post-thaw viability significantly . It was suggested that the microalgae harvested from late exponential phase of cultue was beneficial for cryopreservalion of B . braunii.关键词
葡萄藻/冷冻保藏/冷冻保护剂/程序降温Key words
Botryococcus braunii/ cryoprpservation / Cryoprotectant/ programmed cooling引用本文复制引用
徐玲,刘春朝,李华钟..葡萄藻冷冻保藏的研究[J].工业微生物,2011,41(3):17-20,4.基金项目
中国科学院科研装备研制项目(YZ200947),中国科学院知识创新工程重要方向项目(KSCX2-YW-R-2). （YZ200947）
